無菌液體培養(yǎng)基制備：高效滅菌技術(shù)-濟(jì)南辰宇環(huán)?？萍加邢薰?/title> <link href="/skins/518065/Css/Style.css" rel="stylesheet" type="text/css" /> <script type="text/javascript" src="/skins/518065/js/jquery-3.6.0.min.js"></script> <link rel="shortcut icon" href="/skins/518065/favicon.ico"> <script type="application/ld+json"> { "@context": "https://ziyuan.baidu.com/contexts/cambrian.jsonld", "@id": "http:///Article-3792018.html", "title": "無菌液體培養(yǎng)基制備：高效滅菌技術(shù)", "pubDate": "2024-08-27T13:50:29", "upDate": "2024-08-27T13:51:53" }</script> <script language="javaScript" src="/js/JSChat.js"></script><script language="javaScript">function ChatBoxClickGXH() { DoChatBoxClickGXH('https://chat.chem17.com',521593) }</script><script>!window.jQuery && document.write('<script src="https://public.mtnets.com/Plugins/jQuery/2.2.4/jquery-2.2.4.min.js">'+'</scr'+'ipt>');</script><script type="text/javascript" src="https://chat.chem17.com/chat/KFCenterBox/518065"></script><script type="text/javascript" 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id="ykyu0qm" class="View"> <div id="20gemu8" class="PageView"> <h1 id="inside_rnewtil">無菌液體培養(yǎng)基制備：高效滅菌技術(shù)</h1> <div id="os0isy8" class="inside_rnewtime">更新時間：2024-08-27 點擊次數(shù)：275次</div> <div id="mowwwi0" class="inside_rnewcon"><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">關(guān)鍵詞：液體培養(yǎng)基微生物芽孢細(xì)菌高壓蒸汽滅菌溫度壓力過濾除菌濾菌器濾膜無菌操作紫外線滅菌化學(xué)滅菌<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></strong></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">液體培養(yǎng)基是微生物學(xué)、細(xì)胞生物學(xué)等領(lǐng)域中常用的一種培養(yǎng)基形式，與固體培養(yǎng)基相對應(yīng)，它主要呈液態(tài)，可以通過通氣或振蕩的方式培養(yǎng)微生物或動植物細(xì)胞。液體培養(yǎng)基在配制過程中容易受到細(xì)菌、真菌、芽孢等微生物的污染，從而影響實驗細(xì)胞的生長繁殖。因此需采取有效方式對液體培養(yǎng)基充分滅菌，以保證實驗細(xì)胞的正常生長。</span></strong></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="384" data-backw="578" data-imgfileid="100000385" data-ratio="0.6638888888888889" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6lKjUHwV45xZuWaFXibNFDxBgpPEO86YgQuFbSD3eqCnVSyyMibl4cuvub1To27s585dlIy4uffhXsQ/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="1080" data-original-style="width: 100%;height: auto;" data-index="3" src="https://img51.chem17.com/9/20240827/638603632846090018201.jpg" _width="100%" crossorigin="anonymous" alt="無菌液體培養(yǎng)基制備：高效滅菌技術(shù)"data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 676.997px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">一、高壓蒸汽滅菌</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">高壓蒸汽滅菌是實驗室中常用的滅菌方法之一，適用于耐高溫的物體、器皿及培養(yǎng)基的滅菌。其原理是利用高溫高壓的蒸汽來破壞微生物的細(xì)胞結(jié)構(gòu)，從而達(dá)到滅菌的目的。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">操作步驟如下：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">1.準(zhǔn)備：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">將配制好的液體培養(yǎng)基分裝至合適的容器如三角瓶或試管中，分裝好的培養(yǎng)基用棉塞、紗布或無菌透氣封口膜等包扎密封，放入消毒筐中等待滅菌。注意培養(yǎng)基不要裝得太滿，一般裝量不超過容器的2/3即可，以防在滅菌過程中因容器內(nèi)外壓力不平衡而導(dǎo)致培養(yǎng)基溢出；容器與容器之間應(yīng)留有空隙，不宜太過擁擠，保證蒸汽可以在容器之間流通。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">2.裝鍋：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">將消毒筐放入壓力蒸汽滅菌鍋內(nèi)，關(guān)緊鍋門，防止因鍋內(nèi)蒸汽溫度壓力過高而發(fā)生安全事故。打開蒸汽進(jìn)汽閥門，開始滅菌。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">3.加熱升溫：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">調(diào)節(jié)滅菌鍋的排汽閥，使鍋內(nèi)壓力及溫度逐漸升高至規(guī)定的滅菌要求。在蒸汽壓力為0.105MPa（約1.05大氣壓）時，鍋內(nèi)溫度可達(dá)到121℃。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">4.滅菌：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">在達(dá)到滅菌規(guī)定的溫度和壓力后，保持一段時間，通常為121-124℃，滅菌30min左右，以確保細(xì)菌、真菌、芽孢等微生物被殺滅。記錄滅菌的溫度及時間，做到可追溯。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">5. 降壓取物：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">滅菌結(jié)束后，關(guān)閉蒸汽進(jìn)汽閥門，調(diào)節(jié)排汽閥或通過自然降壓的方式使鍋內(nèi)壓力降至零，打開滅菌鍋門取出培養(yǎng)基。注意從鍋內(nèi)取出物品時應(yīng)戴好手套，防止?fàn)C傷。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"></span><img class="rich_pages wxw-img" data-backh="356.77778" data-backw="280.77778" data-cropselx1="0" data-cropselx2="283" data-cropsely1="0" data-cropsely2="158" data-imgfileid="100000377" data-ratio="1.2701754385964912" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6lKjUHwV45xZuWaFXibNFDxB1BmUnDlQnVJYPPtTRZ2KoPyp577FsjTeE7QaAtlO1FJmAKNpNdLQicw/640?wx_fmt=jpeg&from=appmsg" data-type="gif" data-w="285" data-original-style="background-position: center center;background-size: initial;background-repeat: no-repeat;background-attachment: initial;background-origin: initial;border-width: 1px;border-style: solid;border-color: rgb(221, 221, 221);vertical-align: bottom;background-clip: initial;width: 100%;height: auto;" data-index="4" src="https://img51.chem17.com/9/20240827/638603632847027610341.jpg" _width="100%" crossorigin="anonymous" alt="無菌液體培養(yǎng)基制備：高效滅菌技術(shù)"data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; background-position: center center; background-size: initial; background-repeat: no-repeat; background-attachment: initial; background-origin: initial; border-width: 1px; border-style: solid; border-color: rgb(221, 221, 221); background-clip: initial; width: 676.997px !important; visibility: visible !important;"/><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;"><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;">圖示為高壓蒸汽滅菌</strong></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">二、過濾除菌</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">對于一些不耐熱的液體培養(yǎng)基，如某些植物生長調(diào)節(jié)劑或有機物，溫度過高容易分解，可以采用過濾除菌的方法對培養(yǎng)基進(jìn)行處理。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">操作步驟如下：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">1.準(zhǔn)備：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">選擇合適的濾菌器，通常使用孔徑為0.45μm的薄膜濾菌器，使用前需對濾膜及收集濾液的容器進(jìn)行高溫滅菌處理，防止濾膜及容器上的微生物污染培養(yǎng)基。濾膜應(yīng)定期檢查更換，防止過濾效果降低。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">2.過濾：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">將需要除菌的液體培養(yǎng)基在無菌條件下通過濾菌器進(jìn)行過濾，以去除培養(yǎng)基中的微生物及芽孢。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">3.收集：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"></span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">將過濾后的無菌培養(yǎng)基收集到無菌容器中，并密封保存?zhèn)溆谩?br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img" data-backh="302.77778" data-backw="302.77778" data-cropselx1="0" data-cropselx2="305" data-cropsely1="0" data-cropsely2="170" data-imgfileid="100000376" data-ratio="1" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6lKjUHwV45xZuWaFXibNFDxBptIWeQSeQqZa9J3nQUy2qD00buFLclSY6PYoEmIVbRLRgNfMorLD6Q/640?wx_fmt=jpeg&from=appmsg" data-type="gif" data-w="500" data-original-style="background-position: center center;background-size: initial;background-repeat: no-repeat;background-attachment: initial;background-origin: initial;border-width: 1px;border-style: solid;border-color: rgb(221, 221, 221);vertical-align: bottom;background-clip: initial;width: 100%;height: auto;" data-index="5" src="https://img46.chem17.com/9/20240827/638603632849091419431.jpg" _width="100%" crossorigin="anonymous" alt="無菌液體培養(yǎng)基制備：高效滅菌技術(shù)"data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; background-position: center center; background-size: initial; background-repeat: no-repeat; background-attachment: initial; background-origin: initial; border-width: 1px; border-style: solid; border-color: rgb(221, 221, 221); background-clip: initial; width: 676.997px !important; visibility: visible !important;"/><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;">圖示為過濾除菌用濾膜</span></strong></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">三、其他滅菌方法</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">除了上述兩種常用的滅菌方法外，還有一些其他方法也可以用于液體培養(yǎng)基的滅菌，如紫外線滅菌、化學(xué)滅菌等。但這些方法通常具有局限性，如紫外線滅菌穿透力弱，只適用于空氣及物體表面滅菌；化學(xué)滅菌可能殘留有害物質(zhì)，影響實驗細(xì)胞的正常生長等，因此在實際應(yīng)用中紫外線滅菌及化學(xué)滅菌較少采用。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">四、注意事項</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">1.滅菌時間：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">高壓蒸汽滅菌時間不宜過長或過短，過長可能導(dǎo)致培養(yǎng)基中的營養(yǎng)成分被破壞，過短則可能無法殺滅微生物及芽孢。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">2.溫度控制：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">在滅菌過程中應(yīng)嚴(yán)格控制溫度，確保達(dá)到所需的滅菌溫度并保持足夠的時間。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">3.無菌操作：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">過濾除菌的整個過程要保持無菌操作，避免任何可能的污染。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">4.保存條件：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">滅菌后的培養(yǎng)基應(yīng)盡快使用，如需保存應(yīng)置于適當(dāng)?shù)臏囟认拢ㄈ?0℃以下），并定期檢查是否有污染現(xiàn)象發(fā)生。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" 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