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id="h5h37lj" class="STitle fl"><b>技術(shù)文章</b></span><span id="rjlnbl5" class="Position fr">您的位置：<a href="/">網(wǎng)站首頁(yè)</a> ><a href="/article.html">技術(shù)文章</a> >菌種復(fù)壯方法概覽</span></dt> <dd> <div id="hdtj53r" class="View"> <div id="n53xnbb" class="PageView"> <h1 id="inside_rnewtil">菌種復(fù)壯方法概覽</h1> <div id="53x3rnb" class="inside_rnewtime">更新時(shí)間：2024-08-29 點(diǎn)擊次數(shù)：384次</div> <div id="ppp5jvv" class="inside_rnewcon"><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">關(guān)鍵詞：菌種復(fù)壯純種分離宿主復(fù)壯病毒寄生微生物誘變基因突變淘汰菌絲頂端分離培養(yǎng)基無(wú)菌操作</span><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></strong></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">菌種復(fù)壯指通過淘汰衰老、死亡、變異的細(xì)胞來挑選具有優(yōu)良性狀的細(xì)胞，以此來恢復(fù)菌種原有優(yōu)良性狀和活力的過程。常用方法有純種分離法、宿主復(fù)壯法、誘變法、淘汰法等。針對(duì)不同類型的菌種和復(fù)壯目的，可以采用多種方法。</span></strong></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="302" data-backw="578" data-imgfileid="100000417" data-ratio="0.5231481481481481" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6mfK3kXqvTRDx4zOJgKjR2xfRzKXcic4ua0ibMCX9JjJwGzWLr5SPbcE9owSrZWiahYiclBxl7icpfibt7w/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="1080" data-original-style="width: 100%;height: auto;" data-index="3" src="https://img59.chem17.com/9/20240829/638605357483319623656.jpg" _width="100%" crossorigin="anonymous" alt="菌種復(fù)壯方法概覽"data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 676.997px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">一、純種分離法</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">純種分離法是目前應(yīng)用廣泛的菌種復(fù)壯方法之一，通過挑取單個(gè)性狀優(yōu)良的菌落并進(jìn)行多次分離培養(yǎng)，以去除混雜在菌種中的衰老、死亡和變異的細(xì)胞，從而篩選出具有優(yōu)良性狀的菌種，以達(dá)到復(fù)壯的目的。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">操作步驟如下：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">1.準(zhǔn)備：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">無(wú)菌環(huán)境下，在超凈工作臺(tái)上用無(wú)菌接種針或接種環(huán)從需要復(fù)壯的菌種中挑取一個(gè)性狀優(yōu)良的單菌落。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">2.分離：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">在準(zhǔn)備好的培養(yǎng)皿上劃線分離，注意劃線手法，確保成熟后的菌落便于后續(xù)挑選。操作結(jié)束后將培養(yǎng)皿放在適宜的環(huán)境中培養(yǎng)。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">3.挑選：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">待菌落生長(zhǎng)成熟后，于超凈工作臺(tái)上挑選外觀、顏色、形態(tài)優(yōu)良的菌落，進(jìn)行下一次劃線分離。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">4.重復(fù)操作：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">重復(fù)數(shù)次上述操作至分離出性狀優(yōu)良的純種菌落。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">此外，純種分離法還包括<strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;">稀釋平板法、涂布平板法</strong>等多種方式，可以根據(jù)菌種特性和實(shí)驗(yàn)條件選擇適合的方法來達(dá)到復(fù)壯菌種的目的。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="316" data-backw="474" data-imgfileid="100000418" data-ratio="0.6666666666666666" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6mfK3kXqvTRDx4zOJgKjR2xk7edOhfxicVjpn0TLyicfJpicHFgicbS8GP4Olib44wwJsr5K8rf53QGBfg/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="474" data-original-style="width: 100%;height: auto;" data-index="4" src="https://img59.chem17.com/9/20240829/638605357485038732334.jpg" _width="100%" crossorigin="anonymous" alt="菌種復(fù)壯方法概覽"data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 676.997px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;"><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;">圖示為劃線分離法</strong></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">二、宿主復(fù)壯法</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">宿主復(fù)壯法適用于病毒、立克次氏體等細(xì)胞內(nèi)寄生的微生物。通過將待復(fù)壯的菌種接種到昆蟲或植物的宿主細(xì)胞內(nèi)，利用宿主細(xì)胞內(nèi)復(fù)雜的代謝環(huán)境，提高菌種的活力，從而達(dá)到復(fù)壯的目的。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">操作步驟如下：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">1.準(zhǔn)備：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">準(zhǔn)備昆蟲或植物的宿主細(xì)胞，在無(wú)菌條件下將宿主細(xì)胞接種到適宜的培養(yǎng)基上。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">2.寄生：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">將病毒等需要復(fù)壯的微生物接種到宿主細(xì)胞內(nèi)，使其寄生并感染宿主細(xì)胞。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">3.挑選：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">培養(yǎng)一段時(shí)間后，觀察宿主細(xì)胞的病變程度，從病變程度嚴(yán)重或已病死的宿主細(xì)胞內(nèi)分離出典型菌株再次感染健康的宿主細(xì)胞。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">4. 重復(fù)操作：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">重復(fù)上述操作至菌種活力得到提高。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="266" data-backw="474" data-cropselx1="0" data-cropselx2="578" data-cropsely1="0" data-cropsely2="324" data-imgfileid="100000419" data-ratio="0.5611814345991561" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6mfK3kXqvTRDx4zOJgKjR2xESn5SzzQansIoaSljszEsltN1UOXsBw2iaUgxLlm2Fn7OrEUoR4OwSg/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="474" data-original-style="width: 578px;height: 324px;" data-index="5" src="https://img59.chem17.com/9/20240829/638605357486600811766.jpg" _width="578px" crossorigin="anonymous" alt="菌種復(fù)壯方法概覽"data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 577.986px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;"><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;">圖示為病毒寄生宿主細(xì)胞</strong></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">三、誘變法</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">通過物理或化學(xué)因素使菌種發(fā)生基因突變，從而篩選出具有優(yōu)良性狀的菌株。誘變法存在一定的風(fēng)險(xiǎn)，可能會(huì)導(dǎo)致菌種發(fā)生不可逆的突變，使用時(shí)需注意誘變劑的劑量或輻射時(shí)間。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">操作步驟如下：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">1.準(zhǔn)備：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">準(zhǔn)備好需要復(fù)壯的菌種培養(yǎng)基，選擇合適的誘變劑，如紫外線、抗生素等。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">2.誘變：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">使用誘變劑處理培養(yǎng)基，使菌種發(fā)生基因突變。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">3.挑選：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">將培養(yǎng)基放在適宜的環(huán)境中至培養(yǎng)結(jié)束，無(wú)菌環(huán)境下挑選出具有優(yōu)良性狀的突變株進(jìn)行復(fù)壯。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="323" data-backw="578" data-imgfileid="100000421" data-ratio="0.5592592592592592" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6mfK3kXqvTRDx4zOJgKjR2xXgWI8VR768ibVmI5OBy2za7BSeL8MLGnJLUPwqppFCy69RjnZQx9icdw/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="1080" data-original-style="width: 100%;height: auto;" data-index="6" src="https://img59.chem17.com/9/20240829/638605357488163395564.jpg" _width="100%" crossorigin="anonymous" alt="菌種復(fù)壯方法概覽"data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 676.997px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;"><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;">圖示為用紫外線處理培養(yǎng)皿</strong></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">四、淘汰法</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">淘汰法通過對(duì)衰退的菌種進(jìn)行處理，如高溫、低溫的培養(yǎng)環(huán)境，低營(yíng)養(yǎng)的培養(yǎng)基等，淘汰菌種中的衰老、死亡和變異的細(xì)胞，從而篩選出適應(yīng)力強(qiáng)、有活力的細(xì)胞。淘汰法可以有效去除衰退個(gè)體，增強(qiáng)菌種的優(yōu)良性狀。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">操作步驟如下：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">1.準(zhǔn)備：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">無(wú)菌環(huán)境下，將待復(fù)壯的菌種接種到低營(yíng)養(yǎng)的培養(yǎng)基上，或接種到正常的培養(yǎng)基上放在高溫或低溫環(huán)境中培養(yǎng)。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">2.挑選：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">培養(yǎng)周期結(jié)束后，在超凈工作臺(tái)上挑選出適應(yīng)力強(qiáng)、有活力的菌落進(jìn)行下一步的復(fù)壯操作。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-imgfileid="100000422" data-ratio="0.7290780141843972" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6mfK3kXqvTRDx4zOJgKjR2x7iaQCpO48HDa1qaXGGtAzlvrkMiaZjVb93U3rCec21MiaFNbN50iboOMLQ/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="705" data-original-style="" data-index="7" src="https://img59.chem17.com/9/20240829/638605357490507157494.jpg" _width="677px" crossorigin="anonymous" alt="菌種復(fù)壯方法概覽"data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; visibility: visible !important; width: 676.997px !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;"><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;">圖示為通過調(diào)節(jié)溫度改變培養(yǎng)環(huán)境</strong></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">五、其他方法</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">1.菌絲頂端分離：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">無(wú)菌環(huán)境下挑取健壯菌絲體的頂端部分進(jìn)行純化培養(yǎng)，以恢復(fù)菌種原有的優(yōu)良性狀。此方法適用于不產(chǎn)孢子的絲狀菌。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">2.適當(dāng)更換培養(yǎng)基：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">在菌種傳代培養(yǎng)中適當(dāng)改變培養(yǎng)基成分，以刺激菌絲生長(zhǎng)，提高菌種活力。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">3.定期進(jìn)行菌種分離：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">對(duì)生產(chǎn)中應(yīng)用的菌種每1-2年進(jìn)行一次分離復(fù)壯，以保持菌種的優(yōu)良性狀和活力。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">六、注意事項(xiàng)</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">1.</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">復(fù)壯前需對(duì)菌種進(jìn)行鑒定，保證菌種的純度。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">2.</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">復(fù)壯操作過程中要注意無(wú)菌操作，避免培養(yǎng)基或菌種被雜菌污染，保證菌種正常生長(zhǎng)。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">3.</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">應(yīng)對(duì)復(fù)壯后的菌種進(jìn)行性能評(píng)價(jià)，確保其具有穩(wěn)定的優(yōu)良性狀。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">綜上所述，菌種復(fù)壯是一個(gè)綜合性的過程，需要根據(jù)具體情況選擇合適的方法并按操作規(guī)范嚴(yán)格執(zhí)行。通過菌種復(fù)壯，可以恢復(fù)菌種原有的生命活力，提高菌種的生長(zhǎng)和代謝速度，為發(fā)酵生產(chǎn)、科學(xué)研究等提供具有優(yōu)良性狀的菌種。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; 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