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><a href="/article.html">技術(shù)文章</a> >精準(zhǔn)菌種分離與純化的技術(shù)探索與應(yīng)用</span></dt> <dd> <div id="s00ky8w" class="View"> <div id="g0igkyu" class="PageView"> <h1 id="inside_rnewtil">精準(zhǔn)菌種分離與純化的技術(shù)探索與應(yīng)用</h1> <div id="42y0wac" class="inside_rnewtime">更新時間：2024-09-03 點擊次數(shù)：226次</div> <div id="gs2ew80" class="inside_rnewcon"><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">關(guān)鍵詞：菌種分離純化</span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;"> </span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">純菌種</span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;"> </span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">固體培養(yǎng)基</span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;"> </span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">稀釋平板</span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;"> </span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">涂布平板</span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;"> </span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">平板劃線</span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;"> </span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">液體培養(yǎng)基</span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;"> </span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">單細(xì)胞分離</span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;"> </span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">選擇培養(yǎng)分離</span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;"> </span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">菌懸液</span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;"> </span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">培養(yǎng)基</span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;"> </span><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; letter-spacing: 0.034em; visibility: visible;">無菌操作</span><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></strong></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">菌種分離與純化是微生物學(xué)研究中的關(guān)鍵步驟，旨在從復(fù)雜的微生物群體中分離出單一、純凈的菌株，為后續(xù)的科學(xué)研究、工業(yè)應(yīng)用及醫(yī)療診斷提供基礎(chǔ)材料。本文概述了菌種分離與純化的多種方法，包括固體培養(yǎng)基分離法（如稀釋倒平板法、涂布平板法、平板劃線法等）、液體培養(yǎng)基分離法、單細(xì)胞分離法以及選擇培養(yǎng)分離法等。這些方法各有特點，適用于不同類型的微生物和不同的研究需求。此外，對于特殊類型的微生物，如厭氧菌、嗜熱菌等，還需采用特殊的分離與純化策略。</span></strong></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="289" data-backw="578" data-imgfileid="100000451" data-ratio="0.5" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6lwtZsy8Jic7McV22Uk1FkUKpCfwS83yCU2l5sWltVBpKlDe5zMhGtMB7Z5ic4LeicQqceRiajteAiaa9A/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="1080" data-original-style="width: 100%;height: auto;" data-index="3" src="https://img58.chem17.com/9/20240903/638609677576351577330.jpg" _width="100%" crossorigin="anonymous" alt="精準(zhǔn)菌種分離與純化的技術(shù)探索與應(yīng)用" data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 676.997px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">一、菌種分離與純化的定義</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">菌種分離純化是指通過無菌操作技術(shù)，將所需要的純種菌通過一系列方法從菌落或菌群中單獨分離出來達(dá)到純培養(yǎng)狀態(tài)，即只含有單一的微生物種類。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">二、菌種分離與純化的方法<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></strong></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">1. 固體培養(yǎng)基分離法</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">固體培養(yǎng)基分離法是常用的菌種分離與純化方法，主要包括以下幾種：<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; visibility: visible;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">（1）稀釋倒平板法：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px; visibility: visible;">將菌懸液按一定梯度稀釋后，分別取少許不同梯度的菌液（0.5-1.0ml）與熔化的瓊脂培養(yǎng)基搖勻混合，倒入無菌平皿中，待瓊脂凝固后將平板放入恒溫培養(yǎng)箱中倒置培養(yǎng)。如果稀釋得當(dāng)，平板上會出現(xiàn)分散的單個菌落，挑取這些菌落重復(fù)1-2次上述步驟即可獲得純培養(yǎng)。但該方法不適用于熱敏感菌的死亡或好氧菌的純培養(yǎng)。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" visibility:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="248" data-backw="474" data-imgfileid="100000452" data-ratio="0.5232067510548524" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6lwtZsy8Jic7McV22Uk1FkUKHj7o6p8wBgOibLg38rMXlBPiawRA9qJSNVS2letdOlJ0IlJZQac8Kzwg/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="474" data-original-style="width: 100%;height: auto;" data-index="4" src="https://img54.chem17.com/9/20240903/638609677577446330322.jpg" _width="100%" crossorigin="anonymous" alt="精準(zhǔn)菌種分離與純化的技術(shù)探索與應(yīng)用" data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 676.997px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;">圖示為稀釋倒平板法</span></strong><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: var(--articleFontsize); letter-spacing: 0.034em;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">（2）涂布平板法：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; letter-spacing: 0.034em; font-size: 14px;">將熔化的瓊脂培養(yǎng)基倒入無菌平皿中制成平板，待平板冷卻凝固后，將按梯度稀釋的菌懸液加入平板中，每個平板加入0.1ml，并用無菌玻璃涂棒均勻涂布，涂布完成后將平板放入恒溫培養(yǎng)箱中倒置培養(yǎng)。培養(yǎng)結(jié)束后挑取單個菌落進(jìn)行純化。該方法克服了稀釋倒平板法的部分缺點，適用于更多種類的微生物。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="420" data-backw="420" data-imgfileid="100000455" data-ratio="1" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6lwtZsy8Jic7McV22Uk1FkUKEAJic7RNGqgOxxGPHUicsmY6qZ4KcGRmYZFt4VBibGaWRXkDQiaLBOwDQQ/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="420" data-original-style="width: 100%;height: auto;" data-index="5" src="https://img54.chem17.com/9/20240903/638609677578383161209.jpg" _width="100%" crossorigin="anonymous" alt="精準(zhǔn)菌種分離與純化的技術(shù)探索與應(yīng)用" data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 676.997px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;">圖示為涂布平板法</span></strong></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">（3）平板劃線法：</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">用接種環(huán)或接種針沾取少量菌懸液，在無菌平板表面連續(xù)劃線。隨著劃線次數(shù)的增加，微生物細(xì)胞數(shù)量逐漸減少并分散開來，最終形成單個菌落。重復(fù)上述步驟1-2次即可獲得純種菌種。該方法簡便快速，但可能需要多次劃線以確保純培養(yǎng)。常用方法有斜線法、曲線法、方格法、放射法、四格法等。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="325" data-backw="578" data-imgfileid="100000456" data-ratio="0.5625" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6lwtZsy8Jic7McV22Uk1FkUKRLgwYYV6bOwFFurdjywUBzTazVEoBERuRQPO1hQE2h6icSuT7zXJUSA/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="960" data-original-style="width: 100%;height: auto;" data-index="6" src="https://img54.chem17.com/9/20240903/638609677579633527392.jpg" _width="100%" crossorigin="anonymous" alt="精準(zhǔn)菌種分離與純化的技術(shù)探索與應(yīng)用" data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 676.997px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;">圖示為平板劃線法</span></strong><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">2. 液體培養(yǎng)基分離法</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">對于某些在固體培養(yǎng)基上難以生長的微生物，可以采用液體培養(yǎng)基進(jìn)行分離與純化。常用的方法是稀釋法，即將需要培養(yǎng)的微生物在液體培養(yǎng)基中按梯度稀釋，以達(dá)到高度稀釋的效果。在同一梯度的多個平行試管中培養(yǎng)后，若大部分試管（一般為超過95%的試管）沒有微生物生長，選擇有微生物生長的試管，其中可能含有純培養(yǎng)物。但該方法只能分離出數(shù)量占優(yōu)勢的微生物種類。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="289" data-backw="578" data-imgfileid="100000457" data-ratio="0.5009259259259259" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6lwtZsy8Jic7McV22Uk1FkUKAOVwtD8F3Hb5HkiavAUHrXWg30uSrEicOh3yHIV6ibHMicswWE9LNeFKPw/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="1080" data-original-style="width: 100%;height: auto;" data-index="7" src="https://img54.chem17.com/9/20240903/638609677580883219223.jpg" _width="100%" crossorigin="anonymous" alt="精準(zhǔn)菌種分離與純化的技術(shù)探索與應(yīng)用" data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 676.997px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;">圖示為液體培養(yǎng)基法</span></strong><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">3. 單細(xì)胞分離法</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">單細(xì)胞分離法是將單個細(xì)胞或個體直接從微生物材料中分離出來進(jìn)行純培養(yǎng)的過程。對于個體較小的微生物如細(xì)菌等，可以采用在顯微操作儀下用顯微針挑取的方法進(jìn)行純培養(yǎng)；對于個體較大的微生物如原生動物等，可以在顯微鏡下用毛細(xì)管提取進(jìn)行純培養(yǎng)；也可以將菌懸液稀釋后制成小液滴，在顯微鏡下挑選只含有一個細(xì)胞的液滴進(jìn)行純培養(yǎng)。該方法通常需要在顯微鏡下進(jìn)行，利用顯微操作儀或毛細(xì)管等工具挑取單個微生物細(xì)胞或孢子進(jìn)行培養(yǎng)。因此該方法對操作技術(shù)要求較高，多用于某些高度專業(yè)化的科學(xué)研究。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="409" data-backw="578" data-cropselx1="0" data-cropselx2="578" data-cropsely1="0" data-cropsely2="503" data-imgfileid="100000459" data-ratio="0.7074074074074074" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_png/jpzIw44Ib6lwtZsy8Jic7McV22Uk1FkUKwRdn9MMmeib5nycTic2XyKIYW480VySFYIdtsMiagNAIWO8KJXiayY6k8w/640?wx_fmt=png&from=appmsg" data-type="jpeg" data-w="1080" data-original-style="width: 100%;height: auto;" data-index="8" src="https://img45.chem17.com/9/20240903/638609677584619944989.jpg" _width="100%" crossorigin="anonymous" alt="精準(zhǔn)菌種分離與純化的技術(shù)探索與應(yīng)用" data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 676.997px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;">圖示為單細(xì)胞分離法</span></strong><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">4. 選擇培養(yǎng)分離法</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">根據(jù)待分離微生物的特點（如營養(yǎng)需求、生長條件等），設(shè)計特定的環(huán)境條件進(jìn)行培養(yǎng)。通過抑制其他微生物的生長或促進(jìn)目標(biāo)微生物的生長，使目標(biāo)微生物在群落中的數(shù)量上升，再通過平板稀釋等方法進(jìn)行純培養(yǎng)分離。這種方法特別適用于從自然界中分離、尋找有用的微生物。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="385" data-backw="578" data-cropselx1="0" data-cropselx2="578" data-cropsely1="0" data-cropsely2="393" data-imgfileid="100000461" data-ratio="0.6075949367088608" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6lwtZsy8Jic7McV22Uk1FkUKiaoXRoc2NOxia1Wiaic1m4hYibnJjGbzqTI4Exa5Ix68kmjpbACibqv3eaMg/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="474" data-original-style="width: 578px;height: 351px;" data-index="9" src="https://img48.chem17.com/9/20240903/638609677586877739502.jpg" _width="578px" crossorigin="anonymous" alt="精準(zhǔn)菌種分離與純化的技術(shù)探索與應(yīng)用" data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 577.986px !important; visibility: visible !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 12px;">圖示為選擇培養(yǎng)分離法</span></strong><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">三、菌種分離與純化的注意事項</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;"><br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">1.</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">在進(jìn)行菌種分離與純化時，必須嚴(yán)格遵守?zé)o菌操作規(guī)范，以防止雜菌的污染。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">2.</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">選擇合適的培養(yǎng)基和培養(yǎng)條件對于成功分離與純化微生物至關(guān)重要。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">3.</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">在挑取單菌落進(jìn)行純化時，應(yīng)反復(fù)進(jìn)行多次以確保獲得真正的純培養(yǎng)物。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><strong style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">4.</span></strong><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">對于某些特殊類型的微生物（如厭氧菌、嗜熱菌等），需要采用特殊的分離與純化方法。<br style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important;"/></span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; clear: both; min-height: 1em; color: rgba(0, 0, 0, 0.9); font-family: " pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-align:="" text-wrap:="" background-color:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><span style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; font-size: 14px;">綜上所述，菌種分離與純化是微生物學(xué)中的一項重要技術(shù)，選擇合適的分離與純化方法并嚴(yán)格遵守?zé)o菌操作規(guī)范，可以成功地從混雜的微生物群體中分離出單一的、純凈的微生物菌株。通過菌種分離與純化，可以獲得具有特定生物學(xué)特性和應(yīng)用價值的微生物資源，為微生物學(xué)領(lǐng)域的發(fā)展做出重要貢獻(xiàn)。</span></p><p style="-webkit-tap-highlight-color: transparent; margin-top: 0px; margin-bottom: 24px; padding: 0px; outline: 0px; max-width: 100%; min-height: 1em; color: rgba(0, 0, 0, 0.9); text-align: center;" pingfang="" helvetica="" hiragino="" sans="" microsoft="" yahei="" font-size:="" letter-spacing:="" text-wrap:="" background-color:="" text-align:="" box-sizing:="" border-box="" overflow-wrap:="" break-word=""><img class="rich_pages wxw-img js_insertlocalimg" data-backh="578" data-backw="578" data-imgfileid="100000450" data-ratio="1" data-s="300,640" data-src="https://mmbiz.qpic.cn/sz_mmbiz_jpg/jpzIw44Ib6lwtZsy8Jic7McV22Uk1FkUKvT2kzCLnxOzibOEicuryanhkE7J49TUvwpaqDUSq104begKEX2hdeRSg/640?wx_fmt=jpeg&from=appmsg" data-type="jpeg" data-w="800" data-original-style="width: 100%;height: auto;" data-index="10" src="https://img48.chem17.com/9/20240903/638609677587815515373.jpg" _width="100%" crossorigin="anonymous" alt="精準(zhǔn)菌種分離與純化的技術(shù)探索與應(yīng)用" data-fail="0" style="-webkit-tap-highlight-color: transparent; margin: 0px; padding: 0px; outline: 0px; max-width: 100%; box-sizing: border-box !important; overflow-wrap: break-word !important; vertical-align: bottom; height: auto !important; width: 676.997px !important; visibility: visible !important;"/></p></div> <div id="i0you0k" class="inside_newsthree"> <span><a href="/article.html">返回列表</a><a href="#">返回頂部</a></span> <div class="00cuw2k" id="clear" ></div> <div> <p>上一篇 : <a href="/Article-3798504.html">菌種鑒定：微生物學(xué)的關(guān)鍵步驟</a></p> <p>下一篇 : <a href="/Article-3794960.html">菌落獨立性與菌群多樣性：微生物生態(tài)學(xué)的雙重視角</a></p></div> </div>  </div> </div> </dd> </dl> </div> </div> <div id="maae0oy" class="clear"></div> <footer>  <div id="kik0eqa" class="foot1"> <div id="geiymmu" class="foot1_in clear clearfix"> <ul 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